The Opal Polaris 780 Reagent Pack is a two-part reaction and part of the Phenoptics 2.0 7-color whole slide workflow. The Reagent Pack contains:
- Opal TSA-DIG, 1 vial
- Opal Polaris 780, 1 vial
- Dimethyl sulfoxide (DMSO), 1 x 100 uL
Users should source primary antibodies and other workflow reagents separately. Pack contains enough reagent to stain 50-slides. Generally, 100-300 µL of Opal Working Solution is required per slide.
Protocol for Opal TSA-DIG reconstitution and dilution:
- Dissolve the Opal TSA-DIG Reagent in 75 uL of DMSO.
- Before each procedure, dilute Opal TSA-DIG in 1X Plus Amplification Diluent for manual staining or 1X Plus Automation Amplification Diluent for automation to make Opal TSA-DIG Working Solution. The recommended dilution ratio is 1:100.
Protocol for Opal Polaris 780 reconstitution and dilution:
- Dissolve the Opal Polaris 780 Reagent in 300 uL of ddH2O.
- Before each procedure, dilute Opal Polaris 780 in Blocking/Ab Diluent to make Opal Polaris 780 Working Solution. The recommended dilution ratio is 1:25.
The Opal Reagent Packs can be utilized for both mono- and multiplex tissue staining and have been configured and validated for imaging on the Vectra® Polaris™ quantitative pathology imaging system.
Note: If you have never used Opal Multiplex IHC Detection Reagents before, we strongly suggest purchasing the full Opal kit, which includes a set of quality controlled reagents that have been optimized to work together.
Opal Multiplex IHC Kits make multiplex methods accessible to anyone who works with standard immunohistochemistry. The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may be selected based on performance, rather than species. The method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.
|Detection Method||MSI, Fluorescence|
|Product Brand Name||Opal|
|Quantity in a Package Amount||1.0 per pack|
|Sample Type||Mouse or Human FFPE|
|Shipping Condition||Blue Ice|
|Unit Size||50 Slides|
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The Mantra™ quantitative pathology workstation, with,inForm® image analysis software, enables easy,visualization, quantification and phenotyping of,multiple types of immune cells simultaneously in intact,FFPE tissue sections for cancer immunology research.,Mantra is a compact workstation where a researcher can phenotype immune cells in situ,in solid tumors using multiplexed biomarkers. Unlike existing flow cytometry and next,generation sequencing methods, which can phenotype and quantify immune cells in,homogenized samples, Mantra similarly does this using images of FFPE tissue sections,while maintaining tissue architecture and morphology. This provides spatial information,which could lead to a better understanding of the role and types of immune cells within,both the tumor and the tumor microenvironment so that new cancer immunotherapy,treatments may be identified and researched.,The Mantra is an integrated workstation incorporating multispectral imaging technology,novel image acquisition and inForm analysis software. It can be used with a variety of,stains including PerkinElmer's Opal™ reagent kits. The Mantra system is a part of,PerkinElmer’s cancer immunology portfolio which includes staining reagents, imaging,systems, and image analysis software.
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