The Opal Polaris 7-Color Manual IHC Detection Kit is part of the Phenoptics 2.0 whole slide workflow and contains:
- 6 reactive fluorophores (Opal Polaris 480, Opal 520, Opal 570, Opal 620, Opal 690, and Opal Polaris 780)
- Spectral DAPI
- 1 X Plus Amplification Diluent
- Blocking/Ab Diluent
- Opal Polymer HRP Ms + Rb
- AR6 Buffer
Users should source primary antibodies and other workflow reagents separately. The Opal 7-Color Manual IHC Kit was optimized for use on the Vectra® Polaris™ quantitative pathology imaging system. Kits contain enough reagents to stain 50-slides at the recommended 1:100 dilution ratio.
What is Opal? Opal Multiplex IHC Kits make multiplex methods accessible to researchers who works with standard immunohistochemistry (IHC). The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may now be selected based on performance, rather than species. The method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.
Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.
Opal will enable you to:
- Measure up to six tissue biomarkers at once
- Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
- Retain spatial cellular context that is lost in flow cytometry
- Confirm single cell co-expression for many biomarkers in one tissue section
- Get more information while conserving precious tissue.
|Detection Method||MSI, Fluorescence|
|Product Brand Name||Opal|
|Quantity in a Package Amount||1.0 per pack|
|Sample Type||Human FFPE|
|Shipping Condition||Blue Ice|
|Species Reactivity||Rabbit and Mouse Primary Antibodies|
|Unit Size||50 slides|
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The Mantra™ quantitative pathology workstation, with,inForm® image analysis software, enables easy,visualization, quantification and phenotyping of,multiple types of immune cells simultaneously in intact,FFPE tissue sections for cancer immunology research.,Mantra is a compact workstation where a researcher can phenotype immune cells in situ,in solid tumors using multiplexed biomarkers. Unlike existing flow cytometry and next,generation sequencing methods, which can phenotype and quantify immune cells in,homogenized samples, Mantra similarly does this using images of FFPE tissue sections,while maintaining tissue architecture and morphology. This provides spatial information,which could lead to a better understanding of the role and types of immune cells within,both the tumor and the tumor microenvironment so that new cancer immunotherapy,treatments may be identified and researched.,The Mantra is an integrated workstation incorporating multispectral imaging technology,novel image acquisition and inForm analysis software. It can be used with a variety of,stains including PerkinElmer's Opal™ reagent kits. The Mantra system is a part of,PerkinElmer’s cancer immunology portfolio which includes staining reagents, imaging,systems, and image analysis software.