Opal Polaris 7 Color Automation IHC Detection Kit

Part Number: NEL871001KT



Detail Information

The Opal Polaris 7-Color Automation IHC Detection Kit is part of the Phenoptics 2.0 whole slide workflow and contains:

  • 6 reactive fluorophores (Opal Polaris 480, Opal 520, Opal 570, Opal 620, Opal 690, and Opal Polaris 780)
  • Spectral DAPI
  • 1X Plus Automation Amplification Diluent
  • Blocking/Ab Diluent
  • Opal Polymer HRP Ms + Rb

Users should source primary antibodies and other workflow reagents separately. The Opal Polaris 7-Color Automation IHC Kit was optimized for use on the Leica Biosystems BOND RX and configured and validated for imaging on the Vectra® Polaris™ quantitative pathology imaging system. Kits contain enough reagents to stain 50-slides at the recommended 1:150 dilution ratio.

Opal will enable you to:

  • Measure up to six tissue biomarkers at once
  • Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
  • Retain spatial cellular context that is lost in flow cytometry
  • Confirm single cell co-expression for many biomarkers in one tissue section
  • Get more information while conserving precious tissue.


Automating Opal staining brings the benefit of:

  • Quality, consistency and reproducibility with every sample
  • Time-savings versus laborious manual process
  • Ease-of-use with a walk-away, high-throughput protocol

 

Specifications

Automation Compatible Yes
Detection Method MSI, Fluorescence
Product Brand Name Opal
Quantity in a Package Amount 1.0 per pack
Sample Type Human FFPE
Shipping Condition Blue Ice
Species Reactivity Rabbit and Mouse Primary Antibodies
Unit Size 50 slides

Resources, Events, & More

Brochure:

Brochure: Phenoptics Portfolio

E-book:

Download Phenoptics Research Solutions E-book

Flyer:

Flyer: Opal Multiplex IHC Detection Kits

Product Note:

Product Note: Mantra Quantitative Pathology Workstation

The Mantra™ quantitative pathology workstation, with,inForm® image analysis software, enables easy,visualization, quantification and phenotyping of,multiple types of immune cells simultaneously in intact,FFPE tissue sections for cancer immunology research.,Mantra is a compact workstation where a researcher can phenotype immune cells in situ,in solid tumors using multiplexed biomarkers. Unlike existing flow cytometry and next,generation sequencing methods, which can phenotype and quantify immune cells in,homogenized samples, Mantra similarly does this using images of FFPE tissue sections,while maintaining tissue architecture and morphology. This provides spatial information,which could lead to a better understanding of the role and types of immune cells within,both the tumor and the tumor microenvironment so that new cancer immunotherapy,treatments may be identified and researched.,The Mantra is an integrated workstation incorporating multispectral imaging technology,novel image acquisition and inForm analysis software. It can be used with a variety of,stains including PerkinElmer's Opal™ reagent kits. The Mantra system is a part of,PerkinElmer’s cancer immunology portfolio which includes staining reagents, imaging,systems, and image analysis software.

Vectra Polaris Automated Quantitative Pathology Imaging System Product Note

Support Pages:

Application Support Knowledgebase: Opal Multiplex IHC Assay FAQs