Opal™ is a practical workflow for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed.
The Opal Reagent Pack contains:
- Opal Reagent, 1 vial
- Dimethyl sulfoxide (DMSO), 1 x 100 uL
Users should source primary antibodies and other workflow reagents separately. Before each procedure, dilute reconstituted Opal Fluorophore in 1X Amplification Diluent (not included) to make Opal Fluorophore Working Solution. Pack contains enough reagent to stain 50-slides with the following dilution ratios:
- 1:100 for manual kits
- 1:150 for automated kits
Generally, 100-300 µL of Opal Working Solution is required per slide.
The Opal Reagent Packs can be utilized for both mono- and multiplex tissue staining and have been configured and validated for imaging on the Vectra® and Mantra™ systems.
Note: If you have never used Opal Multiplex IHC Detection Reagents before, we strongly suggest purchasing the full Opal kit, which includes a set of quality controlled reagents that have been optimized to work together.
Opal Multiplex IHC Kits make multiplex methods accessible to anyone who works with standard immunohistochemistry. The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may be selected based on performance, rather than species. The method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.
|Shipping Condition||Blue Ice|
|Unit Size||1 kit|
|Wave Length||588/616 nm|
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